neurotrophic factor (R&D Systems)
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Neurotrophic Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pm42014405-313-31-34
Average 96 stars, based on 208 article reviews
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Recombinant:Article Title: Massively parallel characterization of CRISPR activator efficacy in human induced pluripotent stem cells and neurons Article Snippet: After nucleofection, cells were immediately and gently plated into new 24-well plates coated with Geltrex (Gibco ). .. Each well contained warmedM2media, constituting of Neurobasal -AMedium (Gibco ), B-27 Supplement (50X, Gibco ), 2-Mercaptoethanol (50 mM), Recombinant Human NT-3 Protein (10 ng/mL, R&D systems), Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serum-free (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM all-trans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ml Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes Article Snippet: Donor provenance and karyotypic integrity were validated using KaryoStat (ThermoFisher). .. To initiate differentiation of cells towards a motor neuron identity, cells were seeded in plates coated with Vitronectin at a seeding density of 100k/cm, maintained in mTeSR Plus media supplemented with Geneticin (500ug/ml) for 48 hrs, and induced for 72hr with hNIL Induction Media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), N2 Supplement (1X, 17502048, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Article Title: ABCA7-dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer’s disease through BDNF/NGFR signaling Article Snippet: Recombinant human Neuropeptide-Y , Sigma-Aldrich , Cat# 5017. .. Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays. Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serumfree (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM alltrans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ ml Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine. Article Snippet: .. Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes Article Snippet: Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies). .. For arrayed imaging experiments, cells were thawed, seeded into 384 well plates coated with poly-l-ornithine (P4957, Sigma-Aldrich) and Laminin-511(RL511-175, Peprotech), and maintained until experimental endpoint in hNIL Maturation media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Article Title: Genome-wide profiling of RNA 2’- O -methylation in neurons and identification of orphan snoRNA targets Article Snippet: .. The cell pellet was resuspended in Cortical Media (CM), which was prepared by mixing equal volumes of DMEM/F12 with HEPES and Neurobasal Medium (Gibco, #21103049) and supplementing with 1X B27 Supplement (Gibco, #17504044), 10 ng/mL Control:Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serum-free (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM all-trans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ml Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays. Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serumfree (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM alltrans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ ml Saline:Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine. Article Snippet: .. Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Injection:Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine. Article Snippet: .. Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Imaging:Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes Article Snippet: Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies). .. For arrayed imaging experiments, cells were thawed, seeded into 384 well plates coated with poly-l-ornithine (P4957, Sigma-Aldrich) and Laminin-511(RL511-175, Peprotech), and maintained until experimental endpoint in hNIL Maturation media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), |
