Review



neurotrophic factor  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    R&D Systems neurotrophic factor
    Neurotrophic Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pm42014405-313-31-34
    Average 96 stars, based on 208 article reviews
    neurotrophic factor - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Massively parallel characterization of CRISPR activator efficacy in human induced pluripotent stem cells and neurons
    Article Snippet: After nucleofection, cells were immediately and gently plated into new 24-well plates coated with Geltrex (Gibco ). .. Each well contained warmedM2media, constituting of Neurobasal -AMedium (Gibco ), B-27 Supplement (50X, Gibco ), 2-Mercaptoethanol (50 mM), Recombinant Human NT-3 Protein (10 ng/mL, R&D systems), Recombinant Human BDNF Protein (10 ng/mL, R&D systems), GlutaMAX Supplement (Gibco , 100x), MEM NonEssential Amino Acids Solution (Gibco ,100x), Rock inhibitor (Stem Cell Technologies, Y-27632, 10 mM) and doxycycline (1 mg/ mL). ..

    Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays
    Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serum-free (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM all-trans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ml Recombinant Human BDNF Protein (R&D Systems, Inc., #248-BDB-250) or complete neurobasal medium supplemented with both 10 μM RA and 50 ng/ml BDNF for an additional 72 h. Cells of control groups were plated in wells of 6-well plates without GTX coating. ..

    Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes
    Article Snippet: Donor provenance and karyotypic integrity were validated using KaryoStat (ThermoFisher). .. To initiate differentiation of cells towards a motor neuron identity, cells were seeded in plates coated with Vitronectin at a seeding density of 100k/cm, maintained in mTeSR Plus media supplemented with Geneticin (500ug/ml) for 48 hrs, and induced for 72hr with hNIL Induction Media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), N2 Supplement (1X, 17502048, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). .. Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies).

    Article Title: ABCA7-dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer’s disease through BDNF/NGFR signaling
    Article Snippet: Recombinant human Neuropeptide-Y , Sigma-Aldrich , Cat# 5017. .. Recombinant human BDNF protein , R&D Systems , Cat#11166-BD. .. LM11A-31 dihydrochloride (CAS: 1243259-19-9) , MedChemExpress , Cat# HY-11015.

    Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays.
    Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serumfree (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM alltrans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ ml Recombinant Human BDNF Protein (R&D Systems, Inc., #248-BDB-250) or complete neurobasal medium supplemented with both 10 μM RA and 50 ng/ml BDNF for an additional 72 h. Cells of control groups were plated in wells of 6-well plates without GTX coating. ..

    Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine.
    Article Snippet: .. Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Recombinant Human BDNF Protein (200 ng in 0.9% saline for local injection, 1 μL per site, CAS NO.:248-BDB; R&D Systems), anti-BDNF neutralizing antibody (0.2 μg in 0.9% saline for local injection, 0.2 μL per site, EMD Millipore), Sketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.:1430202-702, Sigma-Aldrich), R-ketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.: 1430202-69-9, Sigma-Aldrich), and fluoxetine (20 mg kg−1 i.p., diluted in 0.9% saline, CAS NO.:56296-78-7, Sigma-Aldrich). ..

    Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes
    Article Snippet: Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies). .. For arrayed imaging experiments, cells were thawed, seeded into 384 well plates coated with poly-l-ornithine (P4957, Sigma-Aldrich) and Laminin-511(RL511-175, Peprotech), and maintained until experimental endpoint in hNIL Maturation media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), EdU (5-ethynyl-2’-deoxyuridine, A10044, ThermoFisher) and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). ..

    Article Title: Genome-wide profiling of RNA 2’- O -methylation in neurons and identification of orphan snoRNA targets
    Article Snippet: .. The cell pellet was resuspended in Cortical Media (CM), which was prepared by mixing equal volumes of DMEM/F12 with HEPES and Neurobasal Medium (Gibco, #21103049) and supplementing with 1X B27 Supplement (Gibco, #17504044), 10 ng/mL Recombinant Human BDNF Protein (R&D Systems, #248-BD), 10 ng/mL Recombinant Human GDNF Protein (R&D Systems, #212-GD), 10 ng/mL Human NT-3 Recombinant Protein (PeproTech, #450-03), and 1 μg/mL Laminin Mouse Protein (Gibco, #23017015). .. Cells were plated at a density of 13 million cells per 100-mm dish in CM supplemented with 10 μM ROCK inhibitor Y-27632 dihydrochloride (Tocris, #1254).

    Control:

    Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays
    Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serum-free (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM all-trans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ml Recombinant Human BDNF Protein (R&D Systems, Inc., #248-BDB-250) or complete neurobasal medium supplemented with both 10 μM RA and 50 ng/ml BDNF for an additional 72 h. Cells of control groups were plated in wells of 6-well plates without GTX coating. ..

    Article Title: Differentiation of SH-SY5Y Cells into Cortical Neuron-like Cells for Tauopathy Modeling and Seeding Assays.
    Article Snippet: Twenty-four hours after plating, the old medium was aspirated and replaced with CTSTM NeurobasalTM A Medium (Gibco; # A1371201) containing 1% Penicillin–Streptomycin (Thermo Fisher Scientific), 1 × GibcoTM B-27TM Supplement (50X) serumfree (ThermoFisher Scientific, #17,504,044), and 10 μM L-glutamine, hereafter referred to as complete neurobasal medium, and further supplemented with 10 μM alltrans-Retinoic acid (RA; Sigma-Aldrich, # R2625). .. After 72 h, the medium was aspirated and replaced with either complete neurobasal medium supplemented with 50 ng/ ml Recombinant Human BDNF Protein (R&D Systems, Inc., #248-BDB-250) or complete neurobasal medium supplemented with both 10 μM RA and 50 ng/ml BDNF for an additional 72 h. Cells of control groups were plated in wells of 6-well plates without GTX coating. ..

    Saline:

    Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine.
    Article Snippet: .. Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Recombinant Human BDNF Protein (200 ng in 0.9% saline for local injection, 1 μL per site, CAS NO.:248-BDB; R&D Systems), anti-BDNF neutralizing antibody (0.2 μg in 0.9% saline for local injection, 0.2 μL per site, EMD Millipore), Sketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.:1430202-702, Sigma-Aldrich), R-ketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.: 1430202-69-9, Sigma-Aldrich), and fluoxetine (20 mg kg−1 i.p., diluted in 0.9% saline, CAS NO.:56296-78-7, Sigma-Aldrich). ..

    Injection:

    Article Title: Locus Coeruleus-Dorsolateral Septum Projections Modulate Depression-Like Behaviors via BDNF But Not Norepinephrine.
    Article Snippet: .. Drugs were prepared: CNO (1 mg kg−1, i.p., diluted in 0.9% saline; 3 mM, 1 μL per site, CAS NO.: C0832; Sigma-Aldrich), K252a (1 μM, 1 μL per site, CAS NO.:99533-80-9, Sigma-Aldrich), propranolol (5 mM, 1 μL per site, CAS NO.:318-98-9, Sigma-Aldrich), phentolamine (10 mM, 1 μL per site, CAS NO.: 73-05-2, Sigma-Aldrich), Recombinant Human BDNF Protein (200 ng in 0.9% saline for local injection, 1 μL per site, CAS NO.:248-BDB; R&D Systems), anti-BDNF neutralizing antibody (0.2 μg in 0.9% saline for local injection, 0.2 μL per site, EMD Millipore), Sketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.:1430202-702, Sigma-Aldrich), R-ketamine (10 mg kg−1, i.p., diluted in 0.9% saline, CAS NO.: 1430202-69-9, Sigma-Aldrich), and fluoxetine (20 mg kg−1 i.p., diluted in 0.9% saline, CAS NO.:56296-78-7, Sigma-Aldrich). ..

    Imaging:

    Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes
    Article Snippet: Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies). .. For arrayed imaging experiments, cells were thawed, seeded into 384 well plates coated with poly-l-ornithine (P4957, Sigma-Aldrich) and Laminin-511(RL511-175, Peprotech), and maintained until experimental endpoint in hNIL Maturation media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), EdU (5-ethynyl-2’-deoxyuridine, A10044, ThermoFisher) and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). ..



    Similar Products

    96
    R&D Systems neurotrophic factor
    Neurotrophic Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pm42014405-313-31-34
    Average 96 stars, based on 1 article reviews
    neurotrophic factor - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems glial cell line
    Glial Cell Line, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pm42014405-313-39-53
    Average 96 stars, based on 1 article reviews
    glial cell line - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    93
    R&D Systems biotin bdnf
    Biotin Bdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Biotinylated+Protein%2C+CF/bio_rxiv__64898__2026__04__08__717148-34-3-4
    Average 93 stars, based on 1 article reviews
    biotin bdnf - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    96
    R&D Systems recombinant bdnf
    Recombinant Bdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pm41896536-453-116-118
    Average 96 stars, based on 1 article reviews
    recombinant bdnf - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems recombinant human bdnf rhbdnf
    Recombinant Human Bdnf Rhbdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pm41760732-385-12-17
    Average 96 stars, based on 1 article reviews
    recombinant human bdnf rhbdnf - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems recombinant human nt3 rhnt3
    Physicochemical characterization of PEGylated <t>NT3-BDNF</t> nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
    Recombinant Human Nt3 Rhnt3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/pmc13046777-334-21-26
    Average 96 stars, based on 1 article reviews
    recombinant human nt3 rhnt3 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    R&D Systems bdnf
    Physicochemical characterization of PEGylated <t>NT3-BDNF</t> nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).
    Bdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bdnf+protein/Recombinant+Human+BDNF+Protein/bio_rxiv__64898__2026__02__20__707051-221-49-50
    Average 96 stars, based on 1 article reviews
    bdnf - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).

    Journal: Scientific Reports

    Article Title: Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discovery

    doi: 10.1038/s41598-026-40196-0

    Figure Lengend Snippet: Physicochemical characterization of PEGylated NT3-BDNF nanoparticles over 28 days (40 320 min) after formulation. ( A ) Loading efficiency of BDNF (open circles) and NT3 (filled circles) in PEGylated NT3–BDNF nanoparticles, determined by ELISA. ( B ) Representative TEM image of PEGylated NT3–BDNF nanoparticles deposited 24 h after formulation (magnification 50,000x; scale bar is 100 nm), showing a dispersed morphology maintained throughout the 28-day period. ( C ) Time-dependent changes in the hydrodynamic diameter ( d H ) of PEGylated NT3–BDNF nanoparticles measured by MADLS. The peak intensity data (open and filled points) correspond to the same nanoparticle population and reveal a bimodal size distribution, reflecting both smaller and larger particle fractions.The d H values were calculated from the Stokes–Einstein equation at pH 7.4 and ionic strength 0.15 M (293 K). Individual points indicate nanoparticle diameter distributions obtained from eight independent samples. ( D )Zeta potential (ζ) of the nanoparticles measured in PBS buffer and calculated using Henry’s equation at an ionic strength of 0.15 M. Dashed lines serve as visual guides. All syntheses were performed in six replicates, and error bars represent mean ± standard deviation (SD).

    Article Snippet: Unfiltered stock solutions (typically 250 mg L-1) of carrier-free recombinant human BDNF (rhBDNF) (248-N4-250/CF; R&D Systems, Canada), as well as carrier-free recombinant human NT3 (rhNT3) (248-BDB-250/CF; R&D Systems), were prepared by dissolving lyophilized of known concentrations in phosphate-buffered saline (PBS) (pH 7.4 ± 0.2, 0.15 M; Biomed, Lublin, Poland) and storing them for no longer than 2 months at −20 °C.

    Techniques: Formulation, Enzyme-linked Immunosorbent Assay, Zeta Potential Analyzer, Standard Deviation